This fusion transcript results from ∼3 Mb interstitial deletion between these two loci at chromosome 21q22. Among these fusion genes, TMPRSS2:ERG is the most prevalent and the only member detected in the majority of reports. The recurrent gene fusion event in prostate cancer involves an androgen controlled gene, TMPRSS2, and members (ERG, ETV1 and ETV4) of the ETS transcription factor family ( 2, 5, 6). Therefore, there is renewed interest in searching for fusion genes in solid tumors, due to their potential impact on basic research and clinical application as has been demonstrated in chronic myelogenous leukemia (CML) ( 3, 4). This finding has since changed the general view that gene fusions play only a minor role in the pathogenesis of epithelial tumors. Only very limited gene fusion events were discovered in solid tumors, mostly in sarcomas, until the recent discovery of TMPRSS2:ETS fusion genes in prostate cancer ( 2). While gene fusions are common in hematological malignancies, their presence in solid tumors is not as well studied due to several technical and analytic problems related to tumor heterogeneity ( 1). More than 350 gene fusions, as a consequence of chromosome aberrations, have been identified ( 1). The assay should facilitate clinical and basic studies for fusion gene screening in clinical specimens, as it can be readily adapted to include multiple gene loci.Ĭhromosome rearrangements are a characteristic feature of cancer. The ability to detect multiple transcript variants in a single assay is critically dependent on both the primer and probe designs. The assay detected TMPRSS2:ERG fusion transcripts with a detection sensitivity of 1% of cancer cells. Here, we present a simple and sensitive microarray-based assay that is able to simultaneously determine multiple fusion variants with a single RT–PCR in impure RNA specimens. Among all of the reported fusion partners in the ETS gene family, TMPRSS2:ERG is the most prevalent one. Nevertheless, there is a growing interest in the role of fusion genes in common epithelial tumors after the discovery of recurrent TMPRSS2:ETS fusions in prostate cancer. Open the newly created JPEG in Photoshop and follow the Plotting with Photoshop instructions for the appropriate plotter.Studies of gene fusions in solid tumors are not as extensive as in hematological malignancies due to several technical and analytical problems associated with tumor heterogeneity.Under Document Title, rename the document as desired, then click Save on the bottom right corner and choose a location to save your JPEG image.Expand Formats on the left side and select JPEG > Under Settings set Resolution to 200 dpi and set Quality to 100% > click Save on the bottom right corner.Select Options > and click Save on left side under Program > choose JPEG from the Standard save format drop down list.(For example, if your document is 30″ x 10″, make the width 30.5″ and the height 10.5″.) Set the width and height values to 0.5″ greater than the document size, to ensure that clipping or scale reduction does not occur. Otherwise, set the Height as the longer dimension. NOTE: If your image will be 60″ or less, set the Width value as the longer edge of your image.(This will bring up a new window titled PDFCreator 0.9.8.) Input the Width and Height of your document (see note below) under Custom Page Size Dimensions.Choose File > Print (or whatever it takes to get to the Print dialog in your program) and select PDFCreator from the Printer Name drop down list.
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